The government's effort to reduce infant mortality is through the Early Breastfeeding Initiation Program (IMD). The Early Breastfeeding Initiation Program is a program of giving exclusive breastfeeding directly to babies after birth. This is very much needed, because the baby immediately gets nutrition from breast milk (Roesli, 2008). This research is an analytical study with a cross sectional study design which is used to determine the relationship between maternal knowledge and the implementation of IMD. The research was conducted at the Hutagalung Health Center in January-July 2020, where the sample size was 40 people by taking total sampling. Data analysis was performed using bivariate analysis with chi scuare test at α = 5%. The results showed that based on knowledge, the majority of respondents had good knowledge as many as 21 people (52.5%), the majority of respondents implemented IMD as many as 23 people (57.5%). Thus it can be concluded that there is a relationship between maternal knowledge and IMD implementation with test scores. chi square 0.036. It is recommended that the Hutagalung puskesmas be able to carry out the proper management of Early Initiation of Breastfeeding immediately after the baby is born so that the failure of breastfeeding in babies can be reduced and the mother's milk supply is maintained.