Pregnancy is an important event in the life of a woman and her family in general. Diseases experienced during pregnancy will have an adverse impact on the baby. One disease that is currently very feared is the Human Immunodeficiency Virus (HIV). According to the World Health Organization (WHO) this is because there is no vaccine to prevent HIV / AIDS (Acquired Immune Deficiency Syndrome) and the treatment has not yet been found. This type of research is an analytical study with a cross-sectional study design that is used to determine how the relationship between the knowledge of pregnant women about HIVAIDS and attitudes towards Provider Initiated Testing and Counseling (PITC). The research was conducted at the Sigompul Health Center, Humbang Hasundutan Regency in 2020 with a sample of 45 people by taking total sampling. Data analysis was performed using bivariate analysis with chi scuare test at α = 5%. The results showed that based on knowledge, the majority of respondents who had good knowledge had good attitudes as many as 15 respondents (33.34%) and respondents who had poor knowledge, the majority had bad attitudes as many as 11 respondents (24.44%). From the analysis of the relationship between maternal knowledge and attitudes towards the previous PICT using the chi square test at an error rate of 5%, it was found that the value of p = 0.186> α (0.05).